The H&E and IHC staining images were obtained utilizing a microcamera (Leica TCS SP8 MP, Germany)
The H&E and IHC staining images were obtained utilizing a microcamera (Leica TCS SP8 MP, Germany). Isolation of myeloid or Compact disc8+ T cells from tumors or spleen The spleens and tumor masses were removed, homogenized, Diethylstilbestrol and digested with collagenase and hyaluronidase solution (2.5?mg/mL collagenase I, 1?mg/mL collagenase IV, 0.25?mg/mL hyaluronidase IV-S, 300?g/mL DNase…
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