Thereafter, we tested their affinity using bio-layer interferometry (BLI)
Thereafter, we tested their affinity using bio-layer interferometry (BLI). of nanodiscs with sub-nanomolar to nanomolar affinity. Epitope mapping analysis revealed specific recognition of 8 amino acid residues in the exposed helix-4 structure of MSP. Further, we performed kinetics binding analysis between adenosine A2a receptor reconstituted nanodiscs and small molecule antagonist ZM241385 using biND5 immobilized sensor…
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