Next, the chromatin-containing pellet was incubated for 1 h in RT in nuclease incubation buffer (150 mM Hepes, pH 7
Next, the chromatin-containing pellet was incubated for 1 h in RT in nuclease incubation buffer (150 mM Hepes, pH 7.9, 1.5 mM MgCl2, and 150 mM KOAc) supplemented with PIs and benzonase (125 U/ml) to solubilize chromatin-bound proteins. corporation of practical domains in chromatin, making sure the coordination of DNA replication and cotranscriptional procedures. Intro…
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