The membrane was incubated with primary antibody (1:3,000 dilution in PBS with 0

The membrane was incubated with primary antibody (1:3,000 dilution in PBS with 0.1% BSA) at 37?C for 15?min. of AIF despite its discussion with AIF. Movement cytometric analysis exposed that 16E6, however, not 6E6, suppressed apoptotic AIF-induced chromatin degradation (an sign of caspase-independent apoptosis) and staurosporine (STS, a proteins kinase inhibitor)-induced apoptosis. AIF knockdown reduced…
Read more