Mother or father and mutant plasmids were introduced into BL21(DE3) for proteins expression and protein were purified as described [13] with small changes (for 5 min in 4 C to eliminate cells
Mother or father and mutant plasmids were introduced into BL21(DE3) for proteins expression and protein were purified as described [13] with small changes (for 5 min in 4 C to eliminate cells. and mutations close to the VLP site effect epitope framework, we released a previously referred to thermal stabilizing disulfide relationship (R48C/T77C) combined with…
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